FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456156655
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG6742620.14781369352158197TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2005750.062.178891
TCGGAAG2179400.057.0337262
CGGAAGA2402200.051.7305563
AGAGCAC5071250.024.9198977
AGCACAC5507950.022.9848619
GAGCACA5608400.022.5899968
GAAGAGC5603300.022.5833785
TATGCCG1553200.017.12900745-49
CTCGTAT1484000.015.91811540-44
AAGAGCA8096950.015.91369156
TCGTATC1701650.015.73495335-39
CGTATGC1687600.015.6472345-49
TCTCGTA1513950.015.62426640-44
TATCTCG1579050.014.87642940-44
ATGCCGT1788850.014.75348745-49
GGAAGAG9114900.014.2699494
GTCACGA1886150.014.24607425-29
GCCGTCT1852850.014.05906750-54
ATTCGTA1914050.013.94041335-39
ATCTCGT1744250.013.65437440-44
Produced by FastQC (version 0.11.5)