FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371498033
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG3954410.10644497813532165TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1346400.053.785821
TCGGAAG1479250.048.8780442
CGGAAGA1631950.044.168613
AGAGCAC3832500.019.3187717
AGCACAC4142950.017.9411289
GAAGAGC4254000.017.5272775
GAGCACA4235750.017.5223568
TATGCCG994800.015.32838445-49
CTCGTAT980700.013.92238340-44
TCGTATC1105350.013.86528735-39
CGTATGC1125850.013.51581445-49
TCTCGTA1007800.013.51347940-44
TATCTCG1052500.012.8293540-44
ATGCCGT1200000.012.75072845-49
GTCACGA1244550.012.38977825-29
GCCGTCT1244450.012.06895950-54
TGCCGTC1207950.012.01984645-49
AAGAGCA6346750.012.0140296
ATTCGTA1299000.011.79160635-39
ATCTCGT1195600.011.54363540-44
Produced by FastQC (version 0.11.5)