FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371498033
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC4530890.12196269152251474Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1955300.037.0227559
GAGCGTC2236350.032.5187958
ATCGGAA2408400.030.952731
TCGGAAG2558300.028.9559482
AGAGCGT2527650.028.811417
CGGAAGA2765700.026.8310623
AAGAGCG2993150.024.703636
GAAGAGC5382200.014.2539655
CGCCGTA1250450.012.0416655-59
TCGCCGT1335300.011.298202555-59
GCCGTAT1414950.010.78208855-59
GTCGCCG1514700.010.15052455-59
TGGTCGC1587100.09.81359150-54
CGTATCA1556250.09.76853260-64
CCGTATC1491050.09.75535455-59
GGAAGAG8438100.09.4599834
GTGGTCG1703300.09.20879350-54
TCCGATC286200.08.9163213
GGTCGCC1692100.08.83877350-54
GCGTCGT1865850.08.166738510-14
Produced by FastQC (version 0.11.5)