FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316000247
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG5883050.18617232283365906TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1400400.075.359721
TCGGAAG1508300.069.9956052
CGGAAGA1672850.062.9704173
AGAGCAC3551800.030.0462237
AGCACAC3809000.028.0798829
GAGCACA3879700.027.6075128
GAAGAGC3902250.027.4911295
AAGAGCA5696450.019.0722626
TATGCCG1127800.019.02252645-49
CTCGTAT1072950.017.60174640-44
CGTATGC1213900.017.51443545-49
TCGTATC1236300.017.29434635-39
TCTCGTA1093550.017.21448340-44
GGAAGAG6371850.017.1553344
ATGCCGT1306450.016.36802945-49
TATCTCG1140300.016.32690440-44
TGCCGTC1296200.016.21223345-49
GTCACGA1347300.016.01905425-29
GCCGTCT1326950.015.8571550-54
TCGTATG1179750.015.77971640-44
Produced by FastQC (version 0.11.5)