FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316000247
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC6723880.212780846339022Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1954750.053.883829
GAGCGTC2185550.048.2499588
ATCGGAA2367900.045.8010641
TCGGAAG2471850.043.6644132
AGAGCGT2501350.042.4394077
CGGAAGA2657050.040.6184923
AAGAGCG2856000.037.5374156
GAAGAGC4857750.022.5198765
CGCCGTA1317900.015.71380255-59
TCGCCGT1373200.015.02292855-59
GGAAGAG7594700.014.7930474
GCCGTAT1478850.014.104568555-59
GTCGCCG1511450.014.10248455-59
CCGTATC1541150.013.38765655-59
TGGTCGC1624500.013.2986750-54
GGTCGCC1638700.013.0507350-54
CGTATCA1624950.012.84357360-64
GTGGTCG1767450.012.32479250-54
GCGTCGT1901050.011.20472310-14
CAGTACG1922550.011.13067730-34
Produced by FastQC (version 0.11.5)