FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414298207
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1513300.043.568741
TCGGAAG1649350.039.8638232
CGGAAGA1847700.035.537053
AGAGCAC4320850.015.6575247
AGCACAC4657550.014.6640339
GAGCACA4732650.014.365538
GAAGAGC4760200.014.2884555
TATGCCG1091750.012.51694245-49
CTCGTAT1030350.011.38809640-44
CGTATGC1217650.011.15134245-49
TCTCGTA1069550.011.01406340-44
TATCTCG1118000.010.40974240-44
ATGCCGT1331350.010.30674245-49
AAGAGCA7068100.09.9058186
TGCCGTC1343600.09.81806645-49
GCCGTCT1384050.09.72096650-54
TCGTATG1209150.09.44167440-44
ATCTCGT1273650.09.36967140-44
GGAAGAG7983450.08.9243044
CGTCTGA1621200.08.65989315-19
Produced by FastQC (version 0.11.5)