FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428384766
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2386400.016.7219269
GAGCGTC2777450.014.4429138
ATCGGAA2938400.014.2923171
TCGGAAG3098250.013.433432
AGAGCGT3042200.013.2523577
CGGAAGA3364150.012.3720163
AAGAGCG3631050.011.3908246
TCCGATC341400.09.4220473
GAAGAGC6303450.07.0005815
CCGATCT470700.06.95746334
TTCCGAT555700.06.3239772
CGCCGTA1354250.06.06861855-59
TCGCCGT1440400.05.71269855-59
GCCGTAT1507950.05.55385855-59
CTTCCGA694600.05.28670841
GTCGCCG1675300.05.07865355-59
CCGTATC1623400.05.07051255-59
CGTATCA1687700.05.013040560-64
Produced by FastQC (version 0.11.5)