FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410865603
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1335150.037.575211
TCGGAAG1471500.033.996812
CGGAAGA1685300.029.7843343
AGAGCAC4101100.012.7658997
AGCACAC4463750.011.7847559
GAGCACA4517100.011.6640438
GAAGAGC4550900.011.6283935
TATGCCG941100.011.07544145-49
CTCGTAT925300.010.10682840-44
TCTCGTA955800.09.83284940-44
CGTATGC1077700.09.66354245-49
TATCTCG1019800.09.15321840-44
ATGCCGT1176800.08.9470845-49
TGCCGTC1172200.08.77071445-49
GCCGTCT1213650.08.557157550-54
TCGTATG1100300.08.46378940-44
ATCTCGT1166450.08.22863640-44
AAGAGCA6888550.08.0042336
CGTCTTC1449950.07.454530250-54
ACACGTC1452700.07.4081610-14
Produced by FastQC (version 0.11.5)