FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452254389
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG4659580.10303006700063223TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1584350.053.5435521
TCGGAAG1734850.048.859222
CGGAAGA1975500.042.9092563
AGAGCAC4585700.018.967747
AGCACAC4953300.017.6568729
GAAGAGC5059050.017.2817755
GAGCACA5072700.017.2698488
TATGCCG1216400.015.46322645-49
GCGCATC1205400.015.29403935-39
CTCGTAT1165800.014.28385540-44
TAATGCG1325300.014.1912230-34
TCTCGTA1174300.014.01501540-44
CGTATGC1371050.013.5720345-49
TGCGCAT1374750.013.47858135-39
ACCGCGC1032450.013.3561421
ATGCCGT1454700.012.92515245-49
CGCATCT1284450.012.81196335-39
ATGCGCA1453300.012.80993135-39
GCCGTCT1463150.012.60116950-54
AATGCGC1379450.012.4401630-34
Produced by FastQC (version 0.11.5)