FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452254389
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC5416060.11975693617867797Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2506300.033.5795069
GAGCGTC2882650.029.278298
ATCGGAA3057450.027.9068471
AGAGCGT3172800.026.6335167
TCGGAAG3243050.026.317662
CGGAAGA3481100.024.5243263
AAGAGCG3718850.022.9097486
GAAGAGC6606450.013.3116725
ACCGCGC1073200.012.9006891
CGCCGTA1580950.011.54632655-59
TCGCCGT1668150.010.95578255-59
GCCGTAT1777700.010.40935955-59
GTCGCCG1882550.09.89191655-59
TGGTCGC2006600.09.46118750-54
CCGCGCC1460350.09.4273392
CGTATCA1965000.09.38787760-64
CCGTATC1880200.08.98687655-59
GTGGTCG2156600.08.84140150-54
GGAAGAG10379200.08.7916284
CCTTCGT1686100.08.3418831
Produced by FastQC (version 0.11.5)