FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454166453
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC19965930.43961701416110543Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4105000.079.9489759
GAGCGTC4435600.073.914928
ATCGGAA4752000.071.522661
AGAGCGT4808500.068.325567
TCGGAAG4960100.068.201132
CGGAAGA5238500.064.482923
AAGAGCG5441650.061.7730646
GAAGAGC8437750.040.2665755
GGAAGAG12341450.027.880594
AGCGGCG686800.020.74799
CGCCGTA3057050.020.50323355-59
TCGCCGT3140600.019.81832155-59
GCCGTAT3249950.019.31492455-59
GTCGCCG3525450.018.92656155-59
CGTATCA3391350.018.37581360-64
TGGTCGC3623500.018.3596550-54
CCGTATC3306950.018.16356755-59
GGTCGCC3741650.017.65618150-54
GTGGTCG3817400.017.5320750-54
GCGTCGT3930200.016.64256510-14
Produced by FastQC (version 0.11.5)