FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442813052
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC4924990.11122052472834518Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2124300.040.804189
GAGCGTC2445150.035.5243158
ATCGGAA2605300.034.1828421
TCGGAAG2795550.031.8296952
AGAGCGT2816350.031.0145767
CGGAAGA3063400.029.0456733
AAGAGCG3294000.026.8822526
GAAGAGC6168100.014.8346885
GCGCATT498400.013.4568821
CGCATTA430300.013.1917992
CGCCGTA1378750.013.09643355-59
TCGCCGT1451550.012.24096155-59
GCCGTAT1585000.011.49371655-59
GTCGCCG1686000.011.38957255-59
TGGTCGC1796600.010.72465350-54
CGTATCA1765400.010.32219460-64
GTGGTCG1929400.010.05106250-54
GGAAGAG9854950.09.6166944
CCGTATC1687600.09.61095555-59
GCGTCGT2040050.09.44966610-14
Produced by FastQC (version 0.11.5)