FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379058681
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG4218170.11128013184850397TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1554450.052.9840431
TCGGAAG1687400.048.7894362
CGGAAGA1866150.044.059823
AGAGCAC4088000.020.5424547
AGCACAC4391800.019.1864179
GAGCACA4487800.018.77088
GAAGAGC4515450.018.701895
TATGCCG1156450.015.3330445-49
CGGCTAT1248650.014.18227230-34
CTCGTAT1077550.013.95421840-44
CGTATGC1277200.013.76307245-49
TCTCGTA1105600.013.60412240-44
AAGAGCA6628500.013.0847636
ATGCCGT1362250.012.99001345-49
GCCGTCT1395200.012.48161950-54
ACCGGCT1473150.012.16073830-34
TGCCGTC1371500.012.01010245-49
ATCTCGT1286150.011.77441840-44
GGAAGAG7398500.011.739544
GTCACCG1576700.011.46600125-29
Produced by FastQC (version 0.11.5)