FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417105245
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1156750.029.3795171
TCGGAAG1278350.026.5563132
CGGAAGA1489900.022.8006273
TATGCCG806350.010.75561245-49
CGTATGC916700.09.45771645-49
CGGCTAT886800.09.21256530-34
AGAGCAC3964550.09.1445727
TCTCGTA838150.08.9118240-44
AGCACAC4273750.08.5659949
CTCGTAT805500.08.56209240-44
GAGCACA4384450.08.3745988
GATCGGA726100.08.326571
GAAGAGC4413100.08.3169595
ACCGGCT1080000.08.16728630-34
GCCGTCT1073850.08.09795250-54
ATGCCGT1030800.07.994512645-49
ATCTCGT1031300.07.415659440-44
CGTCTGA1248350.07.137889415-19
ACACGTC1260100.07.080564510-14
CACCGGC1268250.07.019008630-34
Produced by FastQC (version 0.11.5)