FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445806236
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT5093570.11425524339233335TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1393100.060.4958461
ATCGGAA2152350.039.2876242
TCGGAAG2305250.036.711713
CGGAAGA2541950.033.34064
TATGCCG1753400.018.70460745-49
CTCGTAT1650950.018.24680940-44
TCTCGTA1687400.017.8578140-44
CGGCTAT1834850.017.84227230-34
CGTATGC1892500.017.24783145-49
AGAGCAC5134700.016.8862848
ATGCCGT1972600.016.6003845-49
GCCGTCT2009150.016.10764150-54
ACCGGCT2051300.016.08256530-34
ATCTCGT1901550.015.93894840-44
GAGCACA5604700.015.5142079
GAAGAGC5656150.015.4292766
GTCACCG2199900.015.05089425-29
AGCACAC5498300.015.0022849
CGTCTGA2212600.014.88531215-19
ACACGTC2243000.014.73542210-14
Produced by FastQC (version 0.11.5)