FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467413896
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1623200.050.766971
TCGGAAG1790900.045.903812
CGGAAGA2045050.040.226843
AGAGCAC4786750.017.6371047
AGCACAC5158200.016.350229
GAAGAGC5292500.016.0612755
GAGCACA5279800.016.0271538
TATGCCG1206000.014.63402845-49
CGGCTAT1289150.013.69850230-34
CTCGTAT1152400.013.44049340-44
CGTATGC1331900.013.14948145-49
TCTCGTA1192900.012.99511440-44
ATGCCGT1464150.012.099400545-49
ACCGGCT1519200.011.71769430-34
GCCGTCT1493950.011.70374750-54
TGCCGTC1455400.011.34027745-49
AAGAGCA7932500.011.0942596
ATCTCGT1428700.010.98124440-44
GTCACCG1666700.010.83441725-29
TCACCGG1571650.010.69071625-29
Produced by FastQC (version 0.11.5)