FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467413896
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC4919490.10524911736898811Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2525300.031.7175359
GAGCGTC2859650.028.16978
ATCGGAA3090100.026.6047531
TCGGAAG3276550.025.0332372
AGAGCGT3292750.024.5625557
CGGAAGA3576550.022.9523283
AAGAGCG3884650.021.079186
GAAGAGC6823600.012.4107355
CGCCGTA1593750.010.63263955-59
TCGCCGT1665100.010.0699155-59
GCCGTAT1786200.09.61694255-59
GTCGCCG1897000.09.06746755-59
TCCGATC383100.08.855743
CGTATCA1978800.08.66773960-64
TGGTCGC2025850.08.63629750-54
CCGTATC1897450.08.4898555-59
GGAAGAG10698450.08.3097934
GTGGTCG2159500.08.1279950-54
GGTCGCC2113350.07.8039350-54
GCGTCGT2404350.07.173226410-14
Produced by FastQC (version 0.11.5)