FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419374921
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1379200.055.5522541
TCGGAAG1512150.050.615092
CGGAAGA1719200.044.4823073
AGAGCAC4173300.018.7727387
AGCACAC4556600.017.2935719
GAGCACA4614850.017.1053288
GAAGAGC4622200.017.0624855
TATGCCG1056750.016.03404245-49
CGTATGC1179100.014.1956645-49
CTCGTAT1009750.013.85848340-44
TCTCGTA1039950.013.49367840-44
GTCACGA1306350.013.12362725-29
ATGCCGT1297750.013.09664345-49
GCCGTCT1325650.012.53228150-54
GATCGGA669750.012.2201421
ACGAGAT1421600.012.03826930-34
TGCCGTC1285200.011.98476845-49
AAGAGCA7054650.011.4886336
CGTCTGA1501300.011.41178315-19
ACACGTC1523650.011.32625810-14
Produced by FastQC (version 0.11.5)