FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421134596
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1826600.030.5764439
GAGCGTC2131700.026.2709458
ATCGGAA2334450.024.649271
TCGGAAG2492300.022.9742952
AGAGCGT2464200.022.8519847
CGGAAGA2700700.021.1431623
AAGAGCG2945950.019.397666
GAAGAGC5682650.010.5366245
CGCCGTA1204000.010.47934655-59
TCGCCGT1282800.09.81980655-59
GCCGTAT1394350.09.17449255-59
GTCGCCG1466600.08.84897955-59
TGGTCGC1602950.08.24987850-54
CGTATCA1582550.08.10008860-64
GTGGTCG1733850.07.683027350-54
CCGTATC1495100.07.50767755-59
GCGTCGT1751650.07.333193310-14
CGTCGTG1791250.07.19129810-14
TCCGATC332300.07.10807853
GGAAGAG9045500.06.99661734
Produced by FastQC (version 0.11.5)