FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509731880
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1427100.024.0296461
TCGGAAG1603350.021.3340282
CGGAAGA1813550.018.8314593
GATCGGA969400.011.9264481
TATGCCG1002300.09.20694545-49
CTCGTAT976550.08.34666840-44
TCTCGTA1007900.08.08561140-44
CGTATGC1154750.08.03665845-49
AGAGCAC4882950.07.64075857
ATGCCGT1272500.07.40924145-49
AGCACAC5307300.07.0804459
GCCGTCT1324100.06.988086750-54
GAGCACA5424850.06.93494138
GAAGAGC5431750.06.9305695
ATCTCGT1250650.06.723747340-44
CGTCTGA1569600.06.09005215-19
ACACGTC1622250.05.949584510-14
CGTCTTC1645500.05.913841250-54
CCGTCTT1677800.05.821587650-54
TGCCGTC1266100.05.737654745-49
Produced by FastQC (version 0.11.5)