FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450732296
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1189000.022.4635931
TCGGAAG1330900.020.119052
CGGAAGA1550150.017.2693523
TATGCCG819500.08.11966645-49
CTCGTAT807750.07.43776940-44
GATCGGA796200.07.31708051
TCTCGTA838000.07.07763440-44
CGTATGC955550.06.97724645-49
AGAGCAC4246800.06.85311177
AGCACAC4613750.06.47611479
ATGCCGT1068750.06.403641745-49
GAAGAGC4683200.06.3247565
GAGCACA4707300.06.3181418
GCCGTCT1084950.06.25718450-54
ATCTCGT1055100.05.81222540-44
GAAGACG944750.05.6195046
TGCCGTC1055900.05.43859245-49
CGTCTGA1305850.05.33839615-19
CGTCTTC1350700.05.25908250-54
TCGTATG976250.05.236702440-44
Produced by FastQC (version 0.11.5)