FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429858234
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2338250.015.1672921
GAGCGTC2433950.014.6000899
ATCGGAA2609250.013.6985022
TCGGAAG2762600.012.9916743
AGAGCGT2823450.012.6813758
CGGAAGA3012200.012.0034434
AAGAGCG3316600.010.8763517
CGCCGTA1261850.08.07346555-59
TCGCCGT1341250.07.644175555-59
AGCGTCG2120400.07.35729749
GCCGTAT1458900.07.133077655-59
GTCGCCG1500350.06.881893655-59
CGTATCA1637950.06.49539960-64
GAAGAGC6023000.06.4489166
TGGTCGC1629800.06.436558250-54
TCCGATC319200.06.27063943
GTGGTCG1775800.05.97921950-54
GTACGTC1873100.05.524961530-34
TACGTCC1960600.05.373039730-34
GTGTACG1994300.05.277941725-29
Produced by FastQC (version 0.11.5)