FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451738944
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG4698050.10399922482662907TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1855050.050.8494761
TCGGAAG1990200.047.332892
CGGAAGA2187100.042.9191063
AGAGCAC4833200.019.9838547
AGCACAC5228350.018.4981149
GAAGAGC5292550.018.2851875
GAGCACA5313700.018.1928068
TATGCCG1359600.014.95838545-49
CGTATGC1489800.013.560595545-49
CTCGTAT1262000.013.4128940-44
TCTCGTA1301550.012.92733240-44
ATGCCGT1604750.012.68772245-49
AAGAGCA7856150.012.5555376
GCCGTCT1631850.012.24777250-54
TGCCGTC1599350.011.74054645-49
GGAAGAG8766350.011.3859434
ATCTCGT1509300.011.28338840-44
CGTCTTC1883200.010.86407250-54
CGTCTGA1924450.010.7374515-19
CCGTCTT1922650.010.62230350-54
Produced by FastQC (version 0.11.5)