FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411367370
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1247300.043.0090751
TCGGAAG1372250.039.2165642
CGGAAGA1571350.034.1551443
AGAGCAC4001000.013.9555097
TATGCCG918250.013.42173545-49
AGCACAC4365000.012.9263029
GAAGAGC4453650.012.6217645
GAGCACA4447800.012.6057758
CTCGTAT918800.012.00378840-44
TCGTATC1049250.011.85013535-39
CGTATGC1044900.011.79491745-49
TCTCGTA956450.011.68891940-44
TATCTCG996700.011.18342340-44
ATGCCGT1146300.010.79456245-49
GATCGGA687450.010.5826511
GCCGTCT1169700.010.54265850-54
GTCACGA1176950.010.499989525-29
ATTCGTA1242250.010.09075635-39
ATCTCGT1147800.09.862782540-44
TGCCGTC1130850.09.61499445-49
Produced by FastQC (version 0.11.5)