FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445678097
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT17020810.38190815556278057TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2672450.0104.7088551
ATCGGAA3322600.084.32392
TCGGAAG3460100.080.929533
CGGAAGA3676350.076.0697864
AGAGCAC6306300.044.500518
GAGCACA6800600.041.342759
GAAGAGC6815950.041.3118936
AAGAGCA9366700.030.2870257
GGAAGAG10308650.027.6853585
TATGCCG2926450.023.29729545-49
CTCGTAT2801700.022.57213440-44
TCTCGTA2828800.022.46609140-44
TCGTATC3057800.022.26529535-39
CGTATGC3060000.022.2506645-49
TATCTCG2864700.022.08737440-44
ATGCCGT3149900.021.4715445-49
GCCGTCT3162600.021.35064150-54
GTCACGA3229500.021.2062625-29
ATCTCGT3044050.020.92796340-44
ATTCGTA3271100.020.88745535-39
Produced by FastQC (version 0.11.5)