FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445678097
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT18090090.40590035996316864Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4129500.067.361061
GAGCGTC4600400.060.2420779
ATCGGAA4746500.058.6900252
AGAGCGT4871050.057.0226448
TCGGAAG4905450.056.7970853
CGGAAGA5159750.054.006024
AAGAGCG5418950.051.338757
GAAGAGC8287550.033.936526
GGAAGAG12109300.023.5460155
CGCCGTA3312550.020.55983755-59
TCGCCGT3401250.020.00790855-59
GCCGTAT3508050.019.35584655-59
GTCGCCG3631600.018.81499155-59
CGTATCA3676050.018.6020860-64
TGGTCGC3727050.018.26794450-54
GTGGTCG3882850.017.67965550-54
TCTTCGC4164300.016.42803830-34
GTCTTCG4176450.016.37261230-34
GCGTCGT4195300.016.2641810-14
CGTCGTG4220200.016.1716510-14
Produced by FastQC (version 0.11.5)