FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398045511
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1343800.042.7386471
TCGGAAG1466000.039.1333662
CGGAAGA1640650.034.9144363
GATCGGA767100.019.1862531
AGAGCAC4009950.014.76853757
TATGCCG999950.014.57793945-49
AGCACAC4344450.013.7198239
GAAGAGC4449150.013.4165855
CTCGTAT1003850.013.342607540-44
GAGCACA4451900.013.3317448
TCGTATC1122300.013.05048835-39
TCTCGTA1041850.013.00901840-44
CGTATGC1116250.012.99934945-49
TATCTCG1101600.012.23498440-44
ATGCCGT1202600.012.05149345-49
GCCGTCT1239500.011.66590350-54
GTCACGA1266050.011.49785525-29
ATCTCGT1234150.011.12531840-44
ATTCGTA1323300.011.10545935-39
CGAATTC1385350.010.80491130-34
Produced by FastQC (version 0.11.5)