FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398045511
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1959550.029.0558019
GAGCGTC2255450.025.3442428
ATCGGAA2400750.024.031371
TCGGAAG2529850.022.8192312
AGAGCGT2579100.022.300967
CGGAAGA2765550.020.8729383
AAGAGCG3046100.018.946256
CGCCGTA1245850.011.412642555-59
TCGCCGT1313300.010.87066255-59
GAAGAGC5563100.010.8241165
TCCGATC315600.010.7307953
GCCGTAT1417400.010.07125155-59
GTCGCCG1473700.09.8183455-59
TGGTCGC1586200.09.20407150-54
CGTATCA1590150.09.13876560-64
GTGGTCG1708500.08.69457950-54
GTACGTC1746650.08.29809730-34
TACGTCC1820700.08.07208430-34
GATCGGA1860200.07.9002731
GTGTACG1868300.07.78812425-29
Produced by FastQC (version 0.11.5)