FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417176906
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC7993720.19161463362499745Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2558200.048.5912369
GAGCGTC2863250.043.4923938
ATCGGAA3016000.042.028731
TCGGAAG3187300.039.6868062
AGAGCGT3217650.038.812587
CGGAAGA3393050.037.1993453
AAGAGCG3660350.034.3006746
GAAGAGC6327200.020.2877565
CGCCGTA1823050.014.77961855-59
TCGCCGT1893650.014.18573355-59
GCCGTAT1995550.013.52237155-59
GGAAGAG9887600.013.370474
GTCGCCG2086600.013.18516755-59
TGGTCGC2182850.012.66201950-54
CGTATCA2163450.012.49116560-64
GTGGTCG2329350.011.99012250-54
CCGTATC2087650.011.73855455-59
GGTCGCC2284450.011.07604750-54
GCGTCGT2494650.011.00000510-14
CGTCGTG2532550.010.888062510-14
Produced by FastQC (version 0.11.5)