FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501476830
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG9159170.18264393192403325TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2184350.078.5304261
TCGGAAG2335050.073.294772
CGGAAGA2595100.065.865173
AGAGCAC5549300.031.1913687
AGCACAC5988000.028.8843239
GAGCACA6069100.028.5688958
GAAGAGC6106250.028.4627465
TATGCCG1822550.020.05121245-49
AAGAGCA8941900.019.6636756
CTCGTAT1717100.018.61576740-44
CGTATGC1981500.018.24593245-49
TCTCGTA1754600.018.2030340-44
GGAAGAG9983450.017.8274214
ATGCCGT2086100.017.3484345-49
TATCTCG1826250.017.2340240-44
GCCGTCT2120850.016.7665550-54
TGCCGTC2080700.016.27644545-49
ATCTCGT1999200.016.03247540-44
TCGTATG1884050.015.84495240-44
ACACGTC2348300.015.68271910-14
Produced by FastQC (version 0.11.5)