FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501476830
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC7754730.15463785236099542Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2597800.055.167299
GAGCGTC2940650.048.8237728
ATCGGAA3269800.047.8006481
TCGGAAG3460000.045.099222
AGAGCGT3339750.043.1262177
CGGAAGA3742050.041.7103353
AAGAGCG4017850.038.811476
AGCGGCG771000.032.4528779
GAGCGGC1082900.023.3130538
GAAGAGC7212450.022.0859495
ATCGGGA698600.021.2936971
AGAGCGG1478500.017.3146257
CGCCGTA1784950.015.63085155-59
TCGCCGT1833500.014.70347355-59
GTCGCCG2175450.014.19272455-59
GGAAGAG11553600.014.1327044
GCCGTAT2011750.013.96745555-59
TGGTCGC2284500.013.26069850-54
CGTATCA2219350.012.63158660-64
GTGGTCG2448500.012.46431450-54
Produced by FastQC (version 0.11.5)