FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486237788
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1263600.050.002111
ATCGGAA1756500.036.1647762
TCGGAAG1908400.033.304953
CGGAAGA2143900.029.7104554
TATGCCG1357750.015.3448945-49
CTCGTAT1326300.014.58687740-44
TCTCGTA1368800.014.20701440-44
CGTATGC1514050.013.71006745-49
TATCTCG1430650.013.56546740-44
AGAGCAC5015800.013.0810648
ATGCCGT1623150.012.83675245-49
GCCGTCT1647450.012.62460250-54
ATCTCGT1604100.012.27389540-44
GAGCACA5496400.012.0295999
GAAGAGC5554400.012.020946
ACACGTC1887550.011.1795510-14
CGTCTGA1895550.011.12310515-19
CGTCTTC1929650.010.90609750-54
CCGTCTT1990500.010.60693850-54
CACGTCT2416350.08.90089610-14
Produced by FastQC (version 0.11.5)