FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486237788
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2864150.022.0443521
GAGCGTC3002950.020.9399859
ATCGGAA3305700.019.2353822
AGAGCGT3407750.018.5612148
TCGGAAG3498500.018.1598153
CGGAAGA3776000.016.8819794
AAGAGCG4068650.015.6495067
AGCGTCG2662350.013.9754129
CGCCGTA1769700.011.17341355-59
TCGCCGT1853150.010.61943655-59
GCCGTAT1969350.010.14587955-59
GTCGCCG2079950.09.70947755-59
GAAGAGC7161550.09.3258046
CGTATCA2157100.09.29812960-64
TGGTCGC2227900.09.15439750-54
GTGGTCG2388500.08.6262250-54
TCCGATC385050.08.1877393
GCGTCGT2481550.07.999014410-14
CGTCGTG2517100.07.890645510-14
GTCGTGT2718650.07.43943410-14
Produced by FastQC (version 0.11.5)