FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468295299
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG10062140.21486741424666744TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2318350.088.564111
TCGGAAG2465600.083.163242
CGGAAGA2740950.074.648343
AGAGCAC5440650.037.961817
AGCACAC5865100.035.1279569
GAAGAGC5927050.034.9480175
GAGCACA5956500.034.686388
AAGAGCA8596950.024.3103286
GGAAGAG9544800.022.083954
TATGCCG2003600.021.59345845-49
GCGCATC1968800.021.2797735-39
TAATGCG2084250.020.70736930-34
CGTATGC2149550.019.89325345-49
CTCGTAT1799450.019.8522840-44
TGCGCAT2125500.019.70275735-39
TCTCGTA1813150.019.37762540-44
ATGCGCA2215800.019.14454735-39
ATGCCGT2252650.019.00336845-49
AATGCGC2160450.018.95625130-34
GCCGTCT2272800.018.4085850-54
Produced by FastQC (version 0.11.5)