FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468295299
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC9060660.19348176288227056Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2631150.065.1785359
GAGCGTC2950600.058.06758
ATCGGAA3214600.056.3537181
TCGGAAG3392500.053.1876832
AGAGCGT3332200.051.5392657
CGGAAGA3684000.049.0388153
AAGAGCG3947150.045.8689966
AGCGGCG776750.037.354239
CGCATTA558350.033.8803142
GCGCATT624300.032.5736161
ATCGGGA688250.029.6003721
GAGCGGC1057450.027.0202628
GAAGAGC6884200.026.7269485
AGAGCGG1432050.020.1385567
CGCCGTA1840800.017.48638755-59
GGAAGAG10974200.017.0450154
TCGCCGT1889800.016.49334555-59
GTCGCCG2252950.015.98800155-59
GCCGTAT2049100.015.81147455-59
TGGTCGC2335500.015.06534950-54
Produced by FastQC (version 0.11.5)