FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434379008
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC12251780.2820527643914137Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3225950.059.7018629
GAGCGTC3537550.054.346098
ATCGGAA3805850.051.461421
AGAGCGT3890900.049.5564587
TCGGAAG3968200.049.0285642
CGGAAGA4205900.046.2650833
AAGAGCG4466600.043.43076
GAAGAGC7280600.027.0445825
GGAAGAG11152350.018.0442124
CGCCGTA2285700.016.36523655-59
TCGCCGT2364000.015.78087855-59
GCCGTAT2476500.015.17404955-59
GTCGCCG2654550.014.71002955-59
CCGTATC2564000.014.45776155-59
TGGTCGC2756000.014.26865950-54
CGTATCA2642800.014.20179860-64
GGTCGCC2861250.013.588757550-54
GTGGTCG2928500.013.51282550-54
GCGTCGT3052150.012.67779810-14
CGTCGTG3086050.012.55826510-14
Produced by FastQC (version 0.11.5)