FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390477635
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC20370670.5216859603239504Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3807600.083.918549
GAGCGTC4094900.078.123618
ATCGGAA4297150.075.3633961
TCGGAAG4469150.072.44782
AGAGCGT4447250.071.976677
CGGAAGA4675300.069.171583
AAGAGCG4933500.065.43056
GAAGAGC7412000.043.900645
GGAAGAG10768550.030.5297224
CGCCGTA3047250.021.17170755-59
TCGCCGT3112000.020.62358555-59
GCCGTAT3224300.020.06130455-59
GTCGCCG3337000.019.55538455-59
TGGTCGC3444150.019.08011250-54
CGTATCA3381000.018.95068760-64
CCGTATC3316550.018.90093655-59
GTGGTCG3596450.018.34508350-54
GGTCGCC3504200.018.27984850-54
GCGTCGT3693750.017.7265610-14
CGTCGTG3726450.017.59824210-14
Produced by FastQC (version 0.11.5)