FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394772037
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2165550.025.789169
GAGCGTC2518500.022.2695268
ATCGGAA2757100.020.5858841
AGAGCGT2814250.020.0158637
TCGGAAG2906300.019.5058772
CGGAAGA3112250.018.2850443
AAGAGCG3319050.017.0936726
GAAGAGC5902400.010.02817255
CGCCGTA1181200.09.499742555-59
TCGCCGT1264000.08.9187555-59
GCCGTAT1397700.08.24924855-59
GTCGCCG1425450.08.07136155-59
CCGTATC1472150.07.640965555-59
TGGTCGC1577000.07.46023350-54
CGTATCA1585850.07.315742560-64
GGTCGCC1596050.07.200400450-54
GTGGTCG1772850.06.7154250-54
GGAAGAG9386350.06.71442944
TCTTCGC1998450.05.885072730-34
TCTCGGT2145850.05.7385745-49
Produced by FastQC (version 0.11.5)