FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420881498
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG8738190.20761639657536096TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2146450.079.584441
TCGGAAG2281950.074.718742
CGGAAGA2503800.067.951973
AGAGCAC4971450.034.5319337
AGCACAC5324050.032.209539
GAAGAGC5404150.031.9734155
GAGCACA5419250.031.690528
AAGAGCA7752800.022.4685926
GGAAGAG8563500.020.5038854
TATGCCG1771950.020.05646345-49
CTCGTAT1592700.018.78815540-44
CGTATGC1878850.018.59372745-49
TCTCGTA1636550.018.2564140-44
ATGCCGT2002100.017.6119345-49
GTCACGA2050450.017.55918525-29
GCCGTCT2026950.017.00057650-54
TGCCGTC2003500.016.7570745-49
ACGAGAT2174000.016.50705330-34
ATCTCGT1852250.016.10849440-44
CGTCTGA2264550.015.80622215-19
Produced by FastQC (version 0.11.5)