FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420881498
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC10957490.2603462031966062Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2860950.059.9644669
GAGCGTC3151800.054.487428
ATCGGAA3381700.051.7601621
AGAGCGT3499300.049.2439087
TCGGAAG3547900.049.2263152
CGGAAGA3751650.046.416833
AAGAGCG4013200.043.2920536
GAAGAGC6682300.026.3916365
GGAAGAG10192950.017.6703854
CGCCGTA2117700.016.97114455-59
TCGCCGT2189550.016.37583755-59
GCCGTAT2298900.015.7198655-59
GTCGCCG2395050.015.08333455-59
CGTATCA2461850.014.60343960-64
TGGTCGC2494800.014.59457250-54
CCGTATC2394350.014.34610155-59
GTGGTCG2643650.013.81888850-54
GGTCGCC2583900.013.468595550-54
GCGTCGT2728900.013.18389710-14
CGTCGTG2760750.013.10898410-14
Produced by FastQC (version 0.11.5)