FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381485156
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG4815480.12622981325123958TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1389750.065.906981
TCGGAAG1512650.060.4959562
CGGAAGA1706400.053.4871443
AGAGCAC3973500.023.4312657
AGCACAC4246200.021.9879449
GAAGAGC4360500.021.497995
GAGCACA4358200.021.4145168
TATGCCG1100600.017.02190245-49
CTCGTAT1042300.015.40597440-44
CGTATGC1206350.015.27614345-49
TCTCGTA1067900.014.97284640-44
AAGAGCA6510500.014.6557976
ATGCCGT1297050.014.42254145-49
GTCACGA1317300.014.3480725-29
TGCCGTC1288400.014.16488345-49
GCCGTCT1324950.013.75513650-54
GGAAGAG7209450.013.3411964
ACGAGAT1442650.013.12682130-34
TCGTATG1202850.013.08448240-44
ATCTCGT1265400.012.65082340-44
Produced by FastQC (version 0.11.5)