FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424083870
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC6436230.15176785667419984Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2558500.038.477789
GAGCGTC2862200.034.521638
ATCGGAA3185850.031.3433931
AGAGCGT3274250.030.2679967
TCGGAAG3333700.029.9577162
CGGAAGA3549400.028.1806473
AAGAGCG3816400.026.1714386
GAAGAGC6513100.015.712525
CGCCGTA1716500.011.78069955-59
TCGCCGT1785500.011.30838755-59
GTCGCCG1906250.010.59054555-59
GCCGTAT1953750.010.47701355-59
GGAAGAG10203100.010.4122024
TGGTCGC2089700.09.82935250-54
CCGTATC2034950.09.82742455-59
CGTATCA2125150.09.6111360-64
GGTCGCC2092000.09.57670350-54
GTGGTCG2249400.09.19143650-54
GCGTCGT2426250.08.31700110-14
CGTCGTG2458850.08.23444210-14
Produced by FastQC (version 0.11.5)