FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396554899
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC5442760.13725110983939703Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2074400.041.76999
GAGCGTC2374250.036.5925068
ATCGGAA2502550.035.0926671
TCGGAAG2663850.033.0880972
AGAGCGT2727900.031.976827
CGGAAGA2913400.030.1706623
AAGAGCG3189500.027.5802176
GAAGAGC5686700.015.9508045
CGCCGTA1338750.013.30667655-59
TCGCCGT1409150.012.632627555-59
TCCGATC312600.012.4915563
GCCGTAT1519300.011.834105555-59
GTCGCCG1575200.011.36445155-59
CCGTATC1612850.010.83044555-59
TGGTCGC1699250.010.67983550-54
CGTATCA1682000.010.66652160-64
GGAAGAG8920000.010.5152554
GGTCGCC1755400.010.09216950-54
GTGGTCG1815050.010.04797250-54
GTACGTC1835250.09.76126330-34
Produced by FastQC (version 0.11.5)