FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398115887
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG4693650.11789657617958862TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1681000.053.929061
TCGGAAG1817750.049.7141532
CGGAAGA2013400.044.9077223
AGAGCAC4386750.020.983927
AGCACAC4739250.019.525599
GAAGAGC4827550.019.2272055
GAGCACA4816200.019.1579698
TATGCCG1261350.014.95507145-49
CTCGTAT1149000.014.08400640-44
TCTCGTA1184950.013.57106240-44
CGTATGC1387800.013.477509545-49
AAGAGCA7062900.013.4272776
ATGCCGT1464750.012.83084745-49
GCCGTCT1508050.012.2703850-54
TGCCGTC1474900.012.17236745-49
GGAAGAG7961150.011.9952564
TCGTATG1319300.011.74176740-44
ATCTCGT1388800.011.72628740-44
CGTCTTC1739150.010.88999450-54
CGTCTGA1790700.010.71415915-19
Produced by FastQC (version 0.11.5)