FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398115887
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC5624860.14128700169154515Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2614200.035.079379
GAGCGTC2904050.031.5752268
ATCGGAA3180100.030.17321
TCGGAAG3323950.028.5424372
AGAGCGT3271750.028.1811247
CGGAAGA3534250.026.7097473
AAGAGCG3802050.024.7143766
GAAGAGC6322450.015.2765395
CGCCGTA1693750.011.027955-59
TCGCCGT1757350.010.57929355-59
GGAAGAG9739200.010.2664244
GCCGTAT1864950.010.10572755-59
GTCGCCG1979700.09.76235955-59
TGGTCGC2093350.09.31898550-54
CCGTATC1938000.09.28569455-59
CGTATCA2024800.09.23920160-64
GGTCGCC2166100.08.76304650-54
GTGGTCG2266900.08.70274850-54
GCGTCGT2474900.07.682397410-14
CGTCGTG2513650.07.59684110-14
Produced by FastQC (version 0.11.5)