FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419360113
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1497850.041.630021
TCGGAAG1638800.037.9814832
CGGAAGA1853600.033.704113
AGAGCAC4364650.014.8386577
AGCACAC4718900.013.7309839
GAGCACA4808800.013.5902448
GAAGAGC4796800.013.5577175
TATGCCG1055400.012.25400945-49
CTCGTAT1022800.011.374351540-44
TCTCGTA1055900.010.93677940-44
CGTATGC1195600.010.7697745-49
ATGCCGT1289650.010.15187645-49
TGCCGTC1301100.09.75720745-49
TCGTATG1179000.09.70017440-44
GCCGTCT1341100.09.56780450-54
AAGAGCA7144450.09.407096
ATCTCGT1267000.09.3056640-44
CGTCTTC1589650.08.42023950-54
GGAAGAG8126400.08.3925954
CCGTCTT1609250.08.22578650-54
Produced by FastQC (version 0.11.5)