FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757157
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAT5800.00317369969.9128941
CTCCAAT6300.00627799059.1261561
TCGTTTC7550.0029337178.5393723
CTTCAAC8350.00705564777.74630361
AGATTTA9500.00272265737.5406164
CTCCAAA8600.009184667.52112051
CTCAAAA10750.00108152537.3539841
CATTTGC11400.00208717516.91131835
CTTTAAT10700.0083883596.71668051
CTTCATT11850.00302704916.67133571
TCAAATT12300.0046251886.4064582
ATCAAAT13650.00189672466.31810761
CTTCCAT12750.00649941436.2004181
CTTTATT16654.2655793E-46.0429961
AGATATT17256.997753E-45.81392434
AAGATTT15200.00652885585.6554623
CTTTCAT15250.0065318085.6552251
ATGATAA16850.00334518385.52680163
TGAAATC15700.0093274835.47535134
Produced by FastQC (version 0.11.5)