FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839611
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT8950.025.4051447
CACGTCT9850.024.5266049
ACACGTC10150.023.1016318
AGCACAC14250.016.4548475
GCACACG14100.016.1259616
AGAGCAC16900.013.8746483
GGCTAAT3650.00122460813.6622681
GAGCACA18050.012.9910214
AAGAGCA29350.08.4749542
AGTCACT6450.07.27098320-24
GAAGAGC33050.07.11311961
TTAATAT12900.00114191966.60976933
GTTGAAT13350.00163410446.4035031
CAAAAGT14850.00537617275.75668431
ACGTCTG11300.05.53324710-14
GTCACTG9105.2750693E-115.309778720-24
AGATGTA17900.00750758735.160564
CACTGTG9401.3460522E-105.14076525-29
AACATTT19400.00341702535.1286572
ACTGTGG11151.8189894E-125.098727725-29
Produced by FastQC (version 0.11.5)