FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266269
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGA9201.7590029E-48.7099121
CTTTGGG9502.4895376E-48.4348621
CTTGAAT11853.7506346E-57.9916031
CTTTAAT17502.3491157E-77.4927871
ATACAAG10750.00105303627.37340646
CTTCGTT11500.00191752166.9679291
CTTTGAA19651.6076992E-66.6729651
AAGTTCG10800.0089619916.6715375
TTTTGTG15651.8560255E-46.4461097
CTTCTGA14755.5616186E-46.42037251
CTTTTAT23306.1087485E-76.25292161
GTTCTCC14050.00225773086.22176841
AACTGCC15000.00529067735.7646636
CTTTTCT25752.1753958E-55.37508441
CTTTGAT19200.00220106155.31172661
CTTTTAA18000.00598523355.2611391
CGTTGAT18250.0070340625.18906831
ATTTTAC19950.00407072765.0567226
CTTTTCG20200.00415105055.04876951
CTGCCGT20200.00415105055.04876951
Produced by FastQC (version 0.11.5)