FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005003403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005003403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501563
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCTT4057.976385E-616.119731
TCAGCGA4650.0067047310.8860412
CTTTGAT7803.0217336E-510.229831
ACAATTT7201.5698586E-410.04266553
CTTTAAT9402.1027045E-610.03196051
CTAAAAT6050.00416220969.5919061
CAACAAT7400.00218049558.8222851
CCCTTTT6650.0090550218.726471
TTTGATT11703.4906327E-58.0349342
CTTTCAT9350.00205659737.7581591
TTGATTT14306.647997E-67.58467053
CTTCTTT16103.359963E-56.7582721
CTTTTTA11000.0099990936.5944351
ATTTAAT12800.0061369116.2338021
ATATTAT15400.0067846465.6348896
Produced by FastQC (version 0.11.5)